A. Less accuracy
B. Low resolution
C. Inconvenient to handle many individual samples
D. Slow in operation
This question was addressed to me in an internship interview.
This intriguing question originated from Amino-Acid Analysers topic in chapter Chromatography of Analytical Instrumentation
The explanation: When ion exchange chromatography is used for amino-acid analysis, high accuracy is obtained. It is inconvenient to handle many individual samples and cleaning of glassware is laborious.